Analysis on changes of purgative biopotency in different processed products of rhubarb / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To determine the difference on purgative biopotency of different processed products of rhubarb and compare rhubarb before and after preparation.</p><p><b>METHOD</b>The prime biopotency of rhubarb reference substance was determined by comparing with the control substance of sennoside B using rat purgative model. Comparing with calibrated rhubarb reference substance, the bioactivity different processed products of rhubarb was determined by the 2,2',2" method.</p><p><b>RESULT</b>The purgative biopotency of crude rhubarb was 825.22 U x g(-1). The purgative biopotency of alcohol-processed rhubarb was 699.05 U x g(-1), The purgative biopotency of steamed rhubarb were 459.76 U x g(-1). Carbonized rhubarb cannot be determinate.</p><p><b>CONCLUSION</b>Crude rhubarb showed a significantly declineing purgative biopotency after being processed, alcohol-processed rhubarb showed less than crude rhubarb and followed by steamed rhubarb. Carbonized rhubarb lost almost all purgative bioactivity.</p>