A new method of calibration and positioning in quantitative analysis of multicomponents by single marker / 药学学报
Acta Pharmaceutica Sinica
;
(12): 1653-1659, 2012.
Article
Dans Chinois
| WPRIM
| ID: wpr-274608
ABSTRACT
This paper aims to establish a new method of calibration and positioning in quantitative analysis of multicomponents by single marker (QAMS), using Shuanghuanglian oral liquid as the research object. Establishing relative correction factors with reference chlorogenic acid to other 11 active components (neochlorogenic acid, cryptochlorogenic acid, cafferic acid, forsythoside A, scutellarin, isochlorogenic acid B, isochlorogenic acid A, isochlorogenic acid C, baicalin and phillyrin wogonoside) in Shuanghuanglian oral liquid by 3 correction methods (multipoint correction, slope correction and quantitative factor correction). At the same time chromatographic peak was positioned by linear regression method. Only one standard uas used to determine the content of 12 components in Shuanghuanglian oral liquid, in stead of needing too many reference substance in quality control. The results showed that within the linear ranges, no significant differences were found in the quantitative results of 12 active constituents in 3 batches of Shuanghuanglian oral liquid determined by 3 correction methods and external standard method (ESM) or standard curve method (SCM). And this method is simpler and quicker than literature methods. The results were accurate and reliable, and had good reproducibility. While the positioning chromatographic peaks by linear regression method was more accurate than relative retention time in literature. The slope and the quantitative factor correction controlling the quality of Chinese traditional medicine is feasible and accurate.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Flavonoïdes
/
Calibrage
/
Médicaments issus de plantes chinoises
/
Chimie
/
Acide chlorogénique
/
Reproductibilité des résultats
/
Chromatographie en phase liquide à haute performance
/
Flavanones
/
Apigénine
/
Glucosides
Type d'étude:
Étude pronostique
langue:
Chinois
Texte intégral:
Acta Pharmaceutica Sinica
Année:
2012
Type:
Article
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