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Establishment and preliminary application of dengue virus envelope domain III IgG antibody capture enzyme-linked immuno-absorbent assay / 中华预防医学杂志
Chinese Journal of Preventive Medicine ; (12): 363-366, 2013.
Article Dans Chinois | WPRIM | ID: wpr-274712
ABSTRACT
<p><b>OBJECTIVE</b>To establish a highly sensitive and specific assay to detect dengue virus (DENV) envelope protein domain III (EDIII) IgG antibody, and to explore its value in the diagnosis and seroepidemiological survey of dengue.</p><p><b>METHODS</b>The DENV EDIII IgG antibody capture ELISA was developed using the recombinant full-length DENV EDIII, which was prepared by Pichia yeast expression system as the capture antigen. The serum samples were collected from the same group of 35 DENV-1 patients of primary infection during disease period in 2006 and their follow-up phase in 2010; and the sensitivity of the assay was compared to that of the commercial Panbio DENV IgG ELISA.</p><p><b>RESULTS</b>The sensitivity of DENV EDIII IgG ELISA in detecting the serum samples from disease period and follow-up phase was 87% (20/23) and 94% (33/35), respectively; whereas the sensitivity of Panbio DENV IgG ELISA was 71% (25/35) and 0, respectively. The sensitivity of DENV EDIII IgG ELISA in detecting the serum samples from both periods was similar, without statistical significance (χ(2) = 0.946, P = 0.331). For serum samples from disease period, the sensitivity of DENV EDIII IgG ELISA was comparable with that of Panbio DENV IgG ELISA (χ(2) = 1.924, P = 0.165). However, DENV EDIII IgG ELISA demonstrated a significantly higher sensitivity than Panbio DENV IgG ELISA in detecting the serum samples from follow-up phase (χ(2) = 62.432, P = 0.000).</p><p><b>CONCLUSION</b>DENV EDIII IgG capture ELISA is highly sensitive in detecting IgG in the serum samples from either disease period or follow-up phase. This method might be a promising alternative for diagnosis and seroepidemiologic survey of dengue.</p>
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Texte intégral: Disponible Indice: WPRIM (Pacifique occidental) Sujet Principal: Virologie / Sang / Immunoglobuline G / Test ELISA / Études séroépidémiologiques / Protéines de l&apos;enveloppe virale / Sensibilité et spécificité / Structure tertiaire des protéines / Dengue / Virus de la dengue Type d'étude: Etude diagnostique Limites du sujet: Humains langue: Chinois Texte intégral: Chinese Journal of Preventive Medicine Année: 2013 Type: Article

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Texte intégral: Disponible Indice: WPRIM (Pacifique occidental) Sujet Principal: Virologie / Sang / Immunoglobuline G / Test ELISA / Études séroépidémiologiques / Protéines de l&apos;enveloppe virale / Sensibilité et spécificité / Structure tertiaire des protéines / Dengue / Virus de la dengue Type d'étude: Etude diagnostique Limites du sujet: Humains langue: Chinois Texte intégral: Chinese Journal of Preventive Medicine Année: 2013 Type: Article