MicroRNAs expression in normal and dissected aortic tissue / 中华心血管病杂志
Chinese Journal of Cardiology
;
(12): 406-410, 2012.
Article
Dans Chinois
| WPRIM
| ID: wpr-275035
ABSTRACT
<p><b>OBJECTIVES</b>To investigate if there is altered microRNAs (miRNAs) expression in aortic dissection (Debakey Type A) and normal aorta tissue.</p><p><b>METHODS</b>Total RNA was exacted from aorta of 5 patients with aortic dissection (AD) and four patients without aortic diseases (NA). miRNAs of the aortic tissues were analyzed by miRNA microarray. Reverse transcription polymerase chain reaction (RT-PCR) was performed to verify the expression of miRNAs in larger sample size (AD = 11 and NA = 9).</p><p><b>RESULTS</b>hsa-miR-146b-5p_st, hsa-miR-19a_st and hsa-miR-505_st were significantly upregulated while hsa-miR-1268_st and hsa-miR-939_st were significantly downregulated [fold change > 2, q-value (%) ≤ 5] in AD group compared with NA group. RT-PCR verified hsa-miR-146b-5p_st miRNAs change in AD group.</p><p><b>CONCLUSIONS</b>Altered miRNAs expression might play an essential role in the pathogenesis of aortic dissection formation and hsa-miR-146b-5p_st might serve as a new diagnosis biomarker of aortic dissection.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
RT-PCR
/
Séquençage par oligonucléotides en batterie
/
Analyse de profil d'expression de gènes
/
MicroARN
/
Génétique
/
/
Métabolisme
Limites du sujet:
Humains
langue:
Chinois
Texte intégral:
Chinese Journal of Cardiology
Année:
2012
Type:
Article
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