Cloning, expression and identification of IL-1ra-Fcepsilon fusion gene / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 1754-1760, 2008.
Article
Dans Chinois
| WPRIM
| ID: wpr-275344
ABSTRACT
Both interleukin-1 and IgE are important in the pathogenic mechanism of the allergy asthma. cDNA of interleukin-1receptor antagonist (IL-1ra) and IgE were cloned and a prokaryotic expression vector IL-1ra-Fcepsilon/pBV220 was constructed. The vector was transformed into Escherichia coli BL21(DE3). The fusion protein was expressed successfully in the form of inclusion body. The recombination protein of IL-1ra-Fcepsilon was highly purified by chromatography of gel filtration and ion exchange, which was identifited by Western blotting. The cell assay showed that the activity of IL-1ra-Fcepsilon was as high as IL-1ra in vitro after refolding. The pharmacokenetic profile of IL-1ra-Fcepsilon and L-1ra was analyzed, and the half time of IL-1ra-Fcepsilon is 4.78 times than that of IL-1ra.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Protéines de fusion recombinantes
/
Immunoglobuline E
/
Fragments Fc des immunoglobulines
/
Clonage moléculaire
/
Escherichia coli
/
Fusion de gènes
/
Antagoniste du récepteur à l'interleukine-1
/
Génétique
/
Métabolisme
langue:
Chinois
Texte intégral:
Chinese Journal of Biotechnology
Année:
2008
Type:
Article
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