Cloning and expression of the pig skeletal muscle musclin gene / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 1248-1252, 2008.
Article
Dans Chinois
| WPRIM
| ID: wpr-275395
ABSTRACT
We found seven tag sequence with high homology in dbEST by using human musclin gene, and got its cDNA sequence, which consists of 651bp and the open reading frame was 54-452 bp detected by RT-PCR, encoding 132 amino acid residue protein. The new gene has high homology with that of human, mouse and rat, the rate is 87.2%, 77.6% and 77.9%, respectively; the gene fragment was cloned into expression vector pGEX-4T-1, and the recombinant was transformed into E. coli BL21. Induced by IPTG, the fusion protein GST-musclin, a 38.59 kD protein was successfully expressed in E. coli BL21 and identified by Western blotting.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Suidae
/
Facteurs de transcription
/
Protéines de fusion recombinantes
/
Données de séquences moléculaires
/
Séquence nucléotidique
/
Cadres ouverts de lecture
/
Séquence d'acides aminés
/
Clonage moléculaire
/
ADN complémentaire
/
Muscles squelettiques
Type d'étude:
Étude pronostique
Limites du sujet:
Animaux
/
Humains
langue:
Chinois
Texte intégral:
Chinese Journal of Biotechnology
Année:
2008
Type:
Article
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