Expression of a shrimp Kunitz-type protease inhibitor in Pichia pastoris and activity analysis / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 500-503, 2008.
Article
Dans Chinois
| WPRIM
| ID: wpr-276093
ABSTRACT
SKPI (shrimp Kunitz-type protease inhibitor) from Marsupenaeus japonicus is a member of serine protease inhibitors which play an important role in the arthropod immunity. To fully understand its function in the innate immunity of shrimp, the skpi gene was cloned into a modified pPIC9K vector with a 6-His tag and expressed by Pichia pastoris GS115. The secretory SKPI was purified from the medium with high purity by using Ni Sepharose High Performance. This results also indicated that the purified SKPI could inhibit the activity of trypsin specifically.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Pichia
/
Protéines recombinantes
/
Inhibiteurs de la sérine protéinase
/
Chimie
/
Inhibiteurs trypsiques
/
Aprotinine
/
Pandalidae
/
Génétique
/
Métabolisme
Limites du sujet:
Animaux
langue:
Chinois
Texte intégral:
Chinese Journal of Biotechnology
Année:
2008
Type:
Article
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