Preparation of Fe3O4-magnetic nanoparticles loaded with adriamycin and its reversal of multidrug resistance in vitro / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 748-751, 2007.
Article
de Zh
| WPRIM
| ID: wpr-276830
Bibliothèque responsable:
WPRO
ABSTRACT
To prepare Fe(3)O(4)-magnetic nanoparticles loaded with adriamycin and investigate the reversal role of drug-loaded nanoparticles in K562 and resistant cell line K562/A02, the drug-loaded nanoparticles were prepared by using mechanical absorption polymerization at different conditions of 4 degrees C or 37 degrees C for 24 or 48 hours. The survival of cells cultured with drug-loaded nanoparticles for 48 hours was detected by MTT assay, then the growth inhibition efficacy of cells was calculated. The results showed that the growth inhibition efficacy of both two cell lines was enhanced with increasing concentration of Fe(3)O(4)-magnetic nanoparticles. The inhibitory ratio of two cell lines obtained at 4 degrees C and for 48 hours was significantly better than that at 37 degrees C and 24 hours. In conclusion, Fe(3)O(4)-magnetic nanoparticles can load adriamycin by using mechanical absorption polymerization, but depended on proper temperature and time. Furthermore, drug-loaded nanoparticles showed an ability reversing multidrug resistance.
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Taille de particule
/
Pharmacologie
/
Vecteurs de médicaments
/
Composés du fer III
/
Doxorubicine
/
Chimie
/
Multirésistance aux médicaments
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Résistance aux médicaments antinéoplasiques
/
Cellules K562
/
Nanoparticules
Limites du sujet:
Humans
langue:
Zh
Texte intégral:
Journal of Experimental Hematology
Année:
2007
Type:
Article