Preparation and identification of monoclonal antibody against UGP2 / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 563-566, 2007.
Article
Dans Chinois
| WPRIM
| ID: wpr-276872
ABSTRACT
The study was aimed to generate monoclonal antibodies (mAbs) against homo sapiens UDP-glucose pyrophosphorylase 2 (UGP2). Normal human liver tissues homogenized, and cytosolic proteins isolated by centrifugation were used to immunize BALB/c mice to generate mAbs by hybridoma technique. The mAbs were identified by ELISA, Western blot, and immunohistochemistry assay. The antibody specificity was confirmed by Uni-ZAP expression library screening. The results indicated that one hybridoma BAD062 secreting specific mAb against UGP2 was established. The Ig subclass of this mAb was IgG(2b) (kappa), and it could be used in ELISA, Western blot, immunohistochemistry assay. The antigen recognized by BAD062 mAb was localized in the hepatocyte cytoplasm, with molecular weight of 56 kD in the cytosolic proteins of human liver tissue. The BAD062 mAb was further confirmed by immunoscreening of Uni-ZAP XR liver cDNA expression library. It is concluded that a hybridoma cell line stably secretes specific mAb against UGP2. This mAb reacted with UGP2 in ELISA, Western blot, immunohistochemistry assay, and would be very useful for the UGP2 studies.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Données de séquences moléculaires
/
Séquence nucléotidique
/
Sécrétions corporelles
/
Allergie et immunologie
/
UTP glucose 1-phosphate uridylyltransferase
/
Hybridomes
/
Foie
/
Métabolisme
/
Souris de lignée BALB C
/
Anticorps monoclonaux
Limites du sujet:
Animaux
/
Humains
langue:
Chinois
Texte intégral:
Journal of Experimental Hematology
Année:
2007
Type:
Article
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