Study on the induced differentiation of bone marrow-derived mesenchymal stem cells into inner ear hair cell-like cells in vitro / 中华耳鼻咽喉头颈外科杂志
Chinese Journal of Otorhinolaryngology Head and Neck Surgery
;
(12): 919-923, 2010.
Article
Dans Chinois
| WPRIM
| ID: wpr-277561
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the feasibility of orientation-induced differentiation of rat bone marrow-derived mesenchymal stem cells (MSC) into cochlear hair cell-like cells in vitro.</p><p><b>METHODS</b>MSC were extracted, cultured and identified. The organ of Corti was isolated from post natal day 1-day 3 Sprague-Dawley rats. Then MSC were co-cultured with organ of Corti for 14 days in vitro. The expression of hair cell markers (myosin VIIa, math1 and calretinin) was detected by immunocytochemical analyses and by reverse transcription-polymerase chain reaction (RT-PCR).</p><p><b>RESULTS</b>The differentiated cells were immunopositive for myosin VIIa, calretinin and math1. And RT-PCR results indicated that these differentiated cells expressed hair cell markers Math1 and myosin VIIa.</p><p><b>CONCLUSIONS</b>MSC cultured in vitro could be successfully induced to differentiate into hair cell-like cells with hair cell molecular markers.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Cellules de la moelle osseuse
/
Différenciation cellulaire
/
Cellules cultivées
/
Rat Sprague-Dawley
/
Biologie cellulaire
/
Cellules souches mésenchymateuses
/
Cellules ciliées auditives internes
Limites du sujet:
Animaux
langue:
Chinois
Texte intégral:
Chinese Journal of Otorhinolaryngology Head and Neck Surgery
Année:
2010
Type:
Article
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