The expression and significance of vascular endothelial growth factor and its receptors in hepatocellular carcinoma cell lines with various metastatic potentialities / 中华肝脏病杂志
Chinese Journal of Hepatology
;
(12): 105-108, 2008.
Article
Dans Chinois
| WPRIM
| ID: wpr-277592
ABSTRACT
<p><b>OBJECTIVES</b>To examine whether or not vascular endothelial growth factor (VEGF) and its receptors were expressed in hepatocellular carcinoma cell lines with various metastatic potentialities.</p><p><b>METHODS</b>Reverse transcription-polymerase chain reaction (RT-PCR), enzyme linked immunosorbent assay (ELISA) and Western blot were employed to study the expressions of VEGFR-1, VEGFR-2, VEGF-A and VEGF-B in four hepatocellular carcinoma cell lines MHCC97-H, MHCC97-L, SMMC7721 and HepG-2 and one normal liver cell line L-02.</p><p><b>RESULTS</b>Three hepatocellular carcinoma cell lines (MHCC97-H, MHCC97-L, SMMC7721) expressed VEGFR-1 mRNA and their proteins. The expression level of VEGFR-1 in MHCC97-H was higher than that in MHCC97-L (P less than 0.05) and the expression level of VEGFR-1 in MHCC97-L was higher than that in SMMC7721 (P less than 0.05). No expression of VEGFR-1 was found in HepG-2 or L-02. All four hepatocellular carcinoma cell lines and the L-02 cell line expressed VEGFR-2 mRNA and the protein, as well as the VEGFR-1 ligands VEGF-A and VEGF-B. The expression level of VEGFR-2 in all tested hepatocellular carcinoma cell lines and normal liver cell line L-02 showed no significant differences (P more than 0.05).</p><p><b>CONCLUSION</b>VEGFR-1 was expressed in 4 hepatocellular carcinoma cell lines with various metastatic potentialities. The expression levels appeared to be positively correlated with the potentialities of metastasis of the hepatocellular carcinoma cell lines. VEGFR-1 may relate to the invasiveness and metastatic potential of the hepatocellular carcinoma.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Anatomopathologie
/
Régulation de l'expression des gènes tumoraux
/
Carcinome hépatocellulaire
/
Récepteur-1 au facteur croissance endothéliale vasculaire
/
Lignée cellulaire tumorale
/
Facteur de croissance endothéliale vasculaire de type A
/
Tumeurs du foie
/
Métabolisme
/
Invasion tumorale
/
Métastase tumorale
Limites du sujet:
Humains
langue:
Chinois
Texte intégral:
Chinese Journal of Hepatology
Année:
2008
Type:
Article
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