Gene clone and its characteristics on band 7-like protein in Plasmodium falciparum FCC1/HN / 中国医学科学院学报

ABSTRACT

<p><b>OBJECTIVE</b>To identify and clone the gene named pfstom gene which encoding the protein belonging to band 7 family and to do primary research on its function.</p><p><b>METHODS</b>Based on the finished data in international public malaria database, coding sequence of pfstom cDNA was obtained by RT-PCR from FCC1/HN. Its phylogenetic profiles and the homogeny were analyzed by some softwares. After Prokaryotic expression, C terminal of Pfstom protein was expressed by Pet30a system. Recombinant Pfstom protein was used to immol/Lunize rabbit and then serum was harvested and the IgG was purified for Western blot.</p><p><b>RESULTS</b>The coding sequence of pfstom is 1,125 bp which encoding 374 amino acids with C-terminal fragment being homogenous to stomatin-like protein which belongs to band 7 family. Phylogenetic profiles analysis revealed its homogeny to stomatin. Western blot showed its stage-specific expression in trophozoite.</p><p><b>CONCLUSION</b>Pfstom belongs to band-7 family. It was expressed specifically in trophozoite in erythrocyte stage of plasmodium falciparum. It was not expressed in ring stage. And it is membrane-related protein. All these results provided the foundation for further research on pfstom.</p>