Effects of leukemia bone marrow stromal cells on resistance of co-cultured HL-60 to idarubicin / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 163-165, 2004.
Article
Dans Chinois
| WPRIM
| ID: wpr-278775
ABSTRACT
To study the role of hematopoietic microenvironment abnormality in development of minimal residual disease and its mechanism, the viability of HL-60 cells was investigated by means of bone marrow stromal cell culture system or co-culture system of bone marrow stromal cell with HL-60 cells and idarubicin (IDA), flow cytometry and ELISA. The results showed that viability of HL-60 cells gradually decreased along with the increase of IDA dose and prolongation of culture time. Amount of HL-60 cells co-cultured with leukemia bone marrow stramal cells was significantly increased as compared with that of the control (P < 0.05). Bone marrow stromal cells or stromal cell conditioned medium reduced the effect of IDA on HL-60 cells in culture. In conclusion, leukemia bone marrow stromal cells contribute to increasing resistance of HL-60 cells to chemotherapeutic agents, and play some role in developing minimal residual disease.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Pharmacologie
/
Physiologie
/
Cellules de la moelle osseuse
/
Idarubicine
/
Survie cellulaire
/
Cellules stromales
/
Cellules HL-60
/
Résistance aux médicaments antinéoplasiques
/
Techniques de coculture
/
Relation dose-effet des médicaments
Limites du sujet:
Humains
langue:
Chinois
Texte intégral:
Journal of Experimental Hematology
Année:
2004
Type:
Article
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