Establishment of a human CD30+ anaplastic large cell lymphoma cell line and its biological characteristics / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 490-494, 2003.
Article
Dans Chinois
| WPRIM
| ID: wpr-278856
ABSTRACT
A CD30+ anaplastic large cell lymphoma (ALCL) cell line was established from the mononuclear cells isolated from pleural effusion of a patient with non-Hodgkin's lymphoma. The cell line's biological characteristics were analyzed. The results showed that the established cell line could survive and proliferate in RPIM 1640 medium; the Wright-Giemsa-stained cells were exactly similar to malignant cells of CD30+ ALCL in morphology, with many diffuse virus granules in cytoplasm; the cytochemical staining of the cells showed the following reactivity pattern positive for acid phosphatase (ACP) and periodic acid-Schiff (PAS), negative for peroxidase (POX), myeloperoxidase (MPO) and platelet peroxidase (PPO). The immunoprofile of the cells was positive for CD45, HLA-DR, CD30 and negative for EMA, CD34, CD38, CD2, CD3, CD4, CD7, CD8, CD10, CD15, CD19 and CD20. The cytogenetic analysis showed complicate d qualitative and quantitative abnormality of chromosomes, without typical t(2;5). It is concluded that the established cell line is CD30+ anaplastic large cell lymphoma cell line.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Anatomopathologie
/
Immunophénotypage
/
Lymphome B diffus à grandes cellules
/
Apoptose
/
Antigènes CD30
/
Herpèsvirus humain de type 4
/
Lignée cellulaire tumorale
/
Allergie et immunologie
/
Génétique
/
Caryotypage
Type d'étude:
Recherche qualitative
Limites du sujet:
Femelle
/
Humains
langue:
Chinois
Texte intégral:
Journal of Experimental Hematology
Année:
2003
Type:
Article
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