Determination of galanthamine in bulb of Lycoris radiata by RP-HPLC / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To establish a method for determination of galanthamine in Lycoris radiata.</p><p><b>METHOD</b>HPLC separation was carried on a column of ODS (4.6 mm x 150 mm, 5 microm), with the mobile phase of phosphate buffer (pH = 3-4)-methanol (93:7) at the flow rate of 1.0 mL x min(-1), and the detection wavelength was set at 289 nm.</p><p><b>RESULT</b>Galanthamine revealed linearity within the range of 3-30 microg x mL(-1) (r = 0.9997), the detection limit was 0.3 ng. The average recovery was 99.5% (RSD = 0.5%).</p><p><b>CONCLUSION</b>The method is easy to operate and the results of the determination are accurate, it can be used to evaluate the quality of L. radiata.</p>