Cloning and expression analysis of 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase gene in Tripterygium wilfordii / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 4378-4383, 2015.
Article
Dans Chinois
| WPRIM
| ID: wpr-279230
ABSTRACT
To clone the 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (TwMCT) full length cDNA from Tripterygium wilfordii, the specific primers were designed according to the transcriptome data and the LCPCR were carried out. After a series of bioinformatics analysis on the TwMCT, the MeJA induced expression content were investigated by real-time fluorescence quantification polymerase chain reaction (RT-qPCR). The result showed that the full of TwMCTcDNA was 1 318 bp nucleotides encoding 311 amino acids. The molecular weight of the deduced TwMCT protein was about 34.14 kDa and the theoretical isoelectric point was 8.65. Result of the RT-qPCR analysis indicated that the content of TwMCT mRNA expression in T. wilfordii suspension cell was rising after treating with MeJA and reached the maximum in 24 h. Cloning and analyzing TwMCT gene from T. wilfordii provided gene element for studying the function and expression regulation of secondary metabolites.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Phylogenèse
/
Protéines végétales
/
Oses phosphates
/
Données de séquences moléculaires
/
Chimie
/
Alignement de séquences
/
Séquence d'acides aminés
/
Clonage moléculaire
/
Structure secondaire des protéines
/
Régulation de l'expression des gènes végétaux
langue:
Chinois
Texte intégral:
China Journal of Chinese Materia Medica
Année:
2015
Type:
Article
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