Full-length sequence of hepatitis B virus isolated from high incidence hepatocellular carcinoma area-Longan county / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
;
(6): 272-276, 2004.
Article
Dans Chinois
| WPRIM
| ID: wpr-279556
ABSTRACT
<p><b>OBJECTIVE</b>To understand full-length sequence of HBV isolated from high incidence hepatocellular carcinoma area-Longan county, Guangxi.</p><p><b>METHODS</b>The nested polymerase chain reaction (nPCR) was used for amplifying the whole HBV DNA in sera of asymptomatic carriers. The products were sequenced by clone sequencing and homological analysis.</p><p><b>RESULTS</b>This isolate contained 3 215 bases. The genotype was C and the serotype was adw. There were 40 point mutations in polymerase gene which made 11 amino acids change. There were 11,2 and 3 point mutations in PreS1, PreS2 and S gene respectively which made 3,1,1, amino acids change. Six point mutations including the double mutations (nt 1762 A to T, 1764 G to A) were found in X gene leading to 4 amino acids change. There were 13 point mutations in C gene which made 2 amino acids change. No mutation was found in a determinant and Pre C. The isolate was quite close to the isolate from Vietnamese in evolution while far from the genotype C isolates from Shanghai, Beijing and Tibet.</p><p><b>CONCLUSION</b>No special sequence was found in the isolate from high incidence hepatocellular carcinoma area, Longan county, Guangxi.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Virologie
/
Données de séquences moléculaires
/
Séquence nucléotidique
/
État de porteur sain
/
Chine
/
Virus de l'hépatite B
/
Épidémiologie
/
Incidence
/
Cadres ouverts de lecture
/
Séquence d'acides aminés
Type d'étude:
Etude d'incidence
/
Étude pronostique
Limites du sujet:
Adulte
/
Humains
/
Mâle
Pays comme sujet:
Asie
langue:
Chinois
Texte intégral:
Chinese Journal of Experimental and Clinical Virology
Année:
2004
Type:
Article
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