Human anti-HCMV neutralizing Fab antibody generated by phage display library / 中华实验和临床病毒学杂志

ABSTRACT

<p><b>BACKGROUND</b>Cloning recombinant human Fab fragment against HCMV for the purpose of prophylaxis and control of HCMV infection.</p><p><b>METHODS</b>The authors constructed a HCMV phage display library with 2 x 10(6) clones, then used purified HCMV viral lysates to pan the library, then screened by ELISA.</p><p><b>RESULTS</b>Three clones showed positive responses in ELISA, they also showed high specificity in IFA, two of them could neutralize HCMV in neutralizing assays.</p><p><b>CONCLUSION</b>The specific binding of Fab antibodies to HCMV was demonstrated by ELISA, IFA and neutralizing activities. These results provide us the basis for further research of neutralizing recombinant human whole IgG molecule.</p>