The cloning and expression of apoptosis associated gene ANNEXIN A2 induced by p53 gene / 中华医学遗传学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To identify the relationship between p53-dependent apoptosis associated genes and tumor metastasis.</p><p><b>METHODS</b>mRNA differential display (mRNA DD) was adopted for gene cloning after the different metastatic potential lung cancer cell lines were infected by Adv-p53 (a reconstructed adenovirus encoding wild type p53 gene). RT-PCR, Northern blot and Western blot assays were used to confirm the result from mRNA DD.</p><p><b>RESULTS</b>After induction by p53 gene, the ANNEXIN A2 gene had differential expression in the cell lines; its level was down regulated in all the cells infected by Adv-p53 gene, especially in the Anip973 cell lines with high metastatic potential. RT-PCR, Northern blot and Western blot assays confirmed the consequence.</p><p><b>CONCLUSION</b>The experimental data suggest that the ANNEXIN A2 gene may relate to cellular apoptosis induced by p53 gene. The affirmative relationship between ANNEXIN A2 gene and p53 needs further investigation.</p>