Effect of Tongxinluo on the proliferation and differentiation of rat embryonic neural stem cells / 南方医科大学学报
Journal of Southern Medical University
;
(12): 679-683, 2008.
Article
Dans Chinois
| WPRIM
| ID: wpr-280122
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of Tongxinluo in on the proliferation and differentiation of rat embryonic neural stem cells (NSCs).</p><p><b>METHODS</b>NSCs were isolated from 12- to 14-day SD rat embryo and treated with Tongxinluo at different doses, and the proliferation and differentiation of the cells were observed by immunofluorescence staining at different time points.</p><p><b>RESULTS</b>The ratio of embryonic NSCs labeled with nestin decreased soon after Tongxinluo treatment, but increased afterwards. Significant difference was noted in the number of cells labeled with beta-tubulin between Tongxinluo group and the control group 3 and 7 days after the treatment, and also between high-dose and low-dose Tongxinluo groups at 7 days.</p><p><b>CONCLUSION</b>Tongxinluo can induce the proliferation and neuronal differentiation of rat embryonic NSCs, and the effect is related to the dose of Tongxinluo administered.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Pharmacologie
/
Médicaments issus de plantes chinoises
/
Différenciation cellulaire
/
Cellules cultivées
/
Technique d'immunofluorescence
/
Rat Sprague-Dawley
/
Biologie cellulaire
/
Prolifération cellulaire
/
Relation dose-effet des médicaments
/
Cellules souches embryonnaires
Limites du sujet:
Animaux
/
Grossesse
langue:
Chinois
Texte intégral:
Journal of Southern Medical University
Année:
2008
Type:
Article
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