Establishment of a One-Step Real-Time RT-PCR Method for the Detection of Venezuelan Equine Encephalitis Virus / 病毒学报
Chinese Journal of Virology
; (6): 107-113, 2015.
Article
de Zh
| WPRIM
| ID: wpr-280287
Bibliothèque responsable:
WPRO
ABSTRACT
Venezuelan equine encephalitis (VEE) is a zoonotic disease caused by the Venezuelan equine encephalitis virus (VEEV) complex. This disease has not yet been reported in China, and it is therefore essential to establish a rapid and accurate method for detection of the virus in order to prevent and control this disease. In this study, a one-step real-time quantitative RT-PCR method was developed for the detection of the VEEV complex. A pair of specific primers and a Taqman probe were designed corresponding to a conserved region of the VEEV gene nspl, allowing the detection of all known strains of different sub- types of the virus. Using RNA synthesized by in vitro transcription as template, the sensitivity of this method was measured at 3.27 x 10(2) copies/microL. No signal was generated in response to RNA from Chikungunya virus (CHIKV), nor to RNA encoding the nsp1 fragment of Eastern equine encephalitis virus (EE-EV) or Western equine encephalitis virus (WEEV), all of which belong to the same genus as VEEV. This indicates that the method has excellent specificity. These results show that this one-step real-time quantitative RT-PCR method may provide an effective tool for the detection of VEEV in China.
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Virologie
/
ARN viral
/
Chine
/
Classification
/
Amorces ADN
/
RT-PCR
/
Virus de l'encéphalite équine du Venezuela
/
Encéphalomyélite équine du Vénézuéla
/
Génétique
/
Méthodes
Type d'étude:
Diagnostic_studies
Limites du sujet:
Humans
Pays comme sujet:
America do sul
/
Asia
/
Venezuela
langue:
Zh
Texte intégral:
Chinese Journal of Virology
Année:
2015
Type:
Article