Detection of pim-1 mRNA in prostate cancer diagnosis / 中华医学杂志(英文版)
Chinese Medical Journal
;
(24): 1491-1493, 2007.
Article
Dans Anglais
| WPRIM
| ID: wpr-280400
ABSTRACT
<p><b>BACKGROUND</b>Pim-1 plays an important role in the apoptosis, proliferation, differentiation of cancer cells and progression of cancer. In this study we detected the expression of pim-1 mRNA in normal prostate, benign prostatic hyperplasia (BPH), and prostate cancer (PCa) and explored its diagnostic value for PCa.</p><p><b>METHODS</b>The prostate tissues were collected from 23 patients with PCa, 37 patients with BPH, and 3 healthy volunteers. Pim-1 mRNA expression levels in these samples were determined by the quantitative real-time PCR (QRT-PCR). The differences of expression were calculated based on a standard curve.</p><p><b>RESULTS</b>The ratio of pim-1 mRNA to beta-actin in the normal prostate, BPH, and PCa were 1.05 +/- 0.04, 2.57 +/- 0.74 and 4.45 +/-0.63, respectively. The differences among PCa, BPH and NT were significant (P < 0.05, respectively).</p><p><b>CONCLUSION</b>Detecting pim-1 mRNA expression by QRT-PCR provides a reliable metric for the diagnosis of PCa.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Prostate
/
Hyperplasie de la prostate
/
Tumeurs de la prostate
/
ARN messager
/
Réaction de polymérisation en chaîne
/
Sensibilité et spécificité
/
Diagnostic
/
Protéines proto-oncogènes c-pim-1
/
Génétique
/
Métabolisme
Type d'étude:
Etude diagnostique
Limites du sujet:
Adulte très âgé
/
Humains
/
Mâle
langue:
Anglais
Texte intégral:
Chinese Medical Journal
Année:
2007
Type:
Article
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