Effects of FPIL6/IL2 on the differentiation and proliferation of lymphoid in the ML-IC assay / 中国医学科学院学报
Acta Academiae Medicinae Sinicae
;
(6): 30-35, 2002.
Article
Dans Chinois
| WPRIM
| ID: wpr-280970
ABSTRACT
<p><b>OBJECTIVE</b>To develop an in vitro assay that allows the culture and identification of a single human bone marrow progenitor closely related to hematopoietic stem cell, which is more primitive than LTC-IC, and to find an efficient culture conditions for NK-IC expansion.</p><p><b>METHODS</b>Fusion protein IL6/IL2 was reconstructed and expressed in E. coli DH5 alpha. ML-IC was determined by watching if the single cell can give rise to secondary progenitors with both LTC-IC and NK-IC characteristics. LTC-IC frequency was determined by the CFC clonogenic methylcellulose assay. NK-IC frequency was determined by phenotyping CD56 positive NK cells. The effect of FPIL6/IL2 on the expansion of NK-IC was examined by comparing the colony number of NK cells before and after the culture.</p><p><b>RESULTS</b>After the initial 4-week expansion culture, we showed that (25.75 +/- 5.68)% of freshly sorted Lin-/34+/DRdim cells were able to generate functional NK-IC in one or more secondary FPIL6/IL2 cultures, whereas (6.81 +/- 1.97)% in the control. A total of 102 NK-IC cells were present when were cultured for 6-7 weeks in FPIL6/IL2 expansion medium, which was much higher than the 33 NK-IC cells in the control.</p><p><b>CONCLUSION</b>ML-IC assay will prove useful to assess a very primitive hematopoietic cell with multilineage generative capacity. FPIL6/IL2 is capable of initiating and promoting NK-IC expansion greatly in ex vivo cultures in terms of net-conservation and net proliferation.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Pharmacologie
/
Dosage biologique
/
Protéines de fusion recombinantes
/
Cellules souches hématopoïétiques
/
Cellules tueuses naturelles
/
Différenciation cellulaire
/
Division cellulaire
/
Cellules cultivées
/
Interleukine-6
/
Interleukine-2
Type d'étude:
Étude pronostique
Limites du sujet:
Animaux
/
Humains
langue:
Chinois
Texte intégral:
Acta Academiae Medicinae Sinicae
Année:
2002
Type:
Article
Documents relatifs à ce sujet
MEDLINE
...
LILACS
LIS