RRLC-UV-MS fingerprint of Radix Astragali / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To establish a method for detecting the fingerprint of Radix Astragali by RRLC-UV-MS.</p><p><b>METHOD</b>Separation was performed on an Agilent Zorbax Extend C18 column (2.1 mm x 100 mm, 1.8 microm). Gradient elution was performed by the mobile phases consisting of acetonitrile and 0.1% formic acid with the flow rate of 0.3 mL x min(-1), the detection wavelength was set at 254 nm, and the column temperature was 30 degrees C.</p><p><b>RESULT</b>The method of fingerprint analysis on Radix Astragali by RRLC-UV-MS was established. Eleven samples of Radix Astragali were analyzed, the similarities were over 0.92, and seven peaks in the fingerprint were designated.</p><p><b>CONCLUSION</b>The method can be applied to the quality control and studies on chemical constituents of Radix Astragali.</p>