Quantitative analysis of aglycone of momordicoside L from Momordica charantia in different areas by HPLC / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 620-622, 2010.
Article
Dans Chinois
| WPRIM
| ID: wpr-281753
ABSTRACT
<p><b>OBJECTIVE</b>To develop an HPLC for determination of aglycone of momordicoside L in Momordica charantia.</p><p><b>METHOD</b>A Kromasil C18 (4.6 mm x 150 mm, 5 microm) column was used. The mobile phase was acetonitrile-H2O (6436), the flow rate was 1.0 mL x min(-1) and the UV detection wavelength was set 203 nm.</p><p><b>RESULT</b>The calibration curves were linear from of 0.025 microg to 1 microg (r =0.9911), the contents of aglycone of momordicoside L in Shandong, Henan, Hebei, Jiangxi are 0.211, 0.033, 0.013, 0.007 mg x g(-1), respectively.</p><p><b>CONCLUSION</b>The method is simple and reliable for determination of aglycone of momordicoside L in M. charantia.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Saponines
/
Calibrage
/
Chimie
/
Chromatographie en phase liquide à haute performance
/
Momordica charantia
/
Méthodes
langue:
Chinois
Texte intégral:
China Journal of Chinese Materia Medica
Année:
2010
Type:
Article
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