Construction of replication-deficient recombinant adenovirus expressing gag-polDelta and gp140TM genes of human immunodeficiency virus in mice / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
;
(6): 150-153, 2004.
Article
Dans Chinois
| WPRIM
| ID: wpr-281831
ABSTRACT
<p><b>BACKGROUND</b>Construction of replication-deficient recombinant adenovirus expressing gag-pol and env genes of human immunodeficiency virus (HIV) in mice.</p><p><b>METHODS</b>gag-polDelta and gp140TM genes were cloned into shuttle vector pAdTrack-CMV respectively, and then the plasmids containing gag-polDelta or gp140TM gene were cotransformed with the backbone of adenovirus into E.coli BJ5183. Transfections of the recombinants were performed to obtain recombinant adenoviruses. Its immunogenicity was evaluated by testing antibody levels of mice primed with DNA vaccines and boosted with recombinant adenoviruses.</p><p><b>RESULTS</b>The replication-deficient recombinant adenovirus could express Gp140TM, Gag P55 and P24 proteins correctly. The mice primed with DNA vaccines and boosted with recombinant adenoviruses elicited high titer of HIV-1-specific antibody compared with that inoculated with DNA vaccines only.</p><p><b>CONCLUSION</b>Replication-deficient recombinant adenovirus expressing gag-polDelta and gp140TM can elicit high titer HIV-1-specific antibodies.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Recombinaison génétique
/
Transfection
/
Produits du gène env
/
Adenoviridae
/
Protéines de fusion gag-pol
/
VIH-1 (Virus de l'Immunodéficience Humaine de type 1)
/
Vaccins contre le SIDA
/
Vaccins à ADN
/
Allergie et immunologie
/
Produits du gène env du virus de l'immunodéficience humaine
Limites du sujet:
Animaux
langue:
Chinois
Texte intégral:
Chinese Journal of Experimental and Clinical Virology
Année:
2004
Type:
Article
Documents relatifs à ce sujet
MEDLINE
...
LILACS
LIS