Cochlear mitochondrial DNA3867bp deletion in aged mice / 中华医学杂志(英文版)
Chinese Medical Journal
;
(24): 1390-1393, 2002.
Article
Dans Anglais
| WPRIM
| ID: wpr-282177
ABSTRACT
<p><b>OBJECTIVES</b>To study the status of cochlear mitochondrial DNA (mtDNA) and to determine the location of mtDNA deletion in aged mice.</p><p><b>METHODS</b>We detected cochlear mtDNA in 2, 7 - 10 and 17 - 19 month old mice by nested polymerase chain reaction (PCR) and DNA sequencing.</p><p><b>RESULTS</b>mtDNA3867bp deletions were found in the cochleae of aged mice. The deletion occurred within nt9103-nt12970 and were flanked by 15 base pair direct repeats. Comparing the incidence of mtDNA3867bp deletions, 17 - 19 month old mice (7/8) were significantly higher than 7 - 10 month old mice (4/16). The deletion was not observed in 2 month old mice (0/7). The ratio of deleted mtDNA/total mtDNA in 17 - 19 month old mice was higher than in 7 - 10 month old mice (P < 0.001).</p><p><b>CONCLUSION</b>Cochlear mtDNA 3867bp deletion in aged mice may be related to presbycusis.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Phosphorylation oxydative
/
Presbyacousie
/
Vieillissement
/
ADN mitochondrial
/
Données de séquences moléculaires
/
Séquence nucléotidique
/
Délétion de séquence
/
Cochlée
/
Génétique
/
Métabolisme
Limites du sujet:
Animaux
langue:
Anglais
Texte intégral:
Chinese Medical Journal
Année:
2002
Type:
Article
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