Experimental studies on the apoptosis of HL-60 cells induced by Brucea javanica oil emulsion / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the inducing effect of Brucea javanica on the apoptpsis of HL-60 cells.</p><p><b>METHOD</b>HL-60 cells were treated with Brucea javanica 1100, 140, 120 (v/v) respectively for 6 h and DNA agarose gel electrophoresis, flow cytometry, fluorescence microscope, electron microscope were used to observe the apoptosis inducing effect of Brucea javanica.</p><p><b>RESULT</b>DNA ladder was seen in the 140 group. The apoptosis cell percentages of 140 and 120 group were 86.8% and 97% respectively. Cells of 140 group showed obvious apoptosis character under fluorescence microscope. Cells were induced apoptosis in 120 and 140 Brucea javanica under electron microscope.</p><p><b>CONCLUSION</b>120 and 140 Brucea Javanica showed obvious apoptosis inducing effect of HL-60.</p>