Effects of zearalenone on proliferation and apoptosis in MCF-7 cells / 中华预防医学杂志
Chinese Journal of Preventive Medicine
; (12): 328-331, 2005.
Article
de Zh
| WPRIM
| ID: wpr-282337
Bibliothèque responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To explore the effects of zearalenone (ZEA) on proliferation and apoptosis in estrogen-dependent human breast cancer MCF-7 cells and the likely underlying molecular mechanisms.</p><p><b>METHODS</b>Cell viability was determined by MTT assay and cell cycle distribution by cytometry. Apoptosis was detected by Cell Death Detection ELISA and cytometry, respectively. The expressions of bax and bcl-2 were examined using multiple RT-PCR and Western-blot both at mRNA and protein level, respectively.</p><p><b>RESULTS</b>The current study confirmed the previous studies that ZEA could stimulate proliferation in MCF-7 cells with inducing a profound increase in S phase and a modest increase in G(2)/M phase that was accompanied by a decrease in G(0)/G(1) phase. ZEA could inhibit apoptosis in MCF-7 cells following estrogen ablation at a range of concentrations of 2 nmol/L -96 nmol/L. Western blot and RT-PCR analysis revealed that the anti-apoptotic bcl-2 was upregulated at both protein and mRNA level, together with the downregulation of pro-apoptotic bax.</p><p><b>CONCLUSION</b>ZEA should have possessed comparative estrogenic activity and could promote the progression of MCF-7 cells through the cell cycle by a decreasing in the G(0)/G(1) phase and by a significant increasing in S-phase. The pro-proliferative activity of ZEA was due to inhibition of apoptosis through regulation of bax/bcl-2 expression.</p>
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Anatomopathologie
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Pharmacologie
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Zéaralénone
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Tumeurs du sein
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Cycle cellulaire
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Technique de Western
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Apoptose
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Protéines proto-oncogènes c-bcl-2
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RT-PCR
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Lignée cellulaire tumorale
Limites du sujet:
Female
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Humans
langue:
Zh
Texte intégral:
Chinese Journal of Preventive Medicine
Année:
2005
Type:
Article