Two-dimensional electrophoresis and mass spectrometry analysis on effects of trichloroethylene on protein of L-02 liver cells / 中华预防医学杂志
Chinese Journal of Preventive Medicine
;
(12): 175-178, 2005.
Article
Dans Chinois
| WPRIM
| ID: wpr-282366
ABSTRACT
<p><b>OBJECTIVE</b>To study the effects of trichloroethylene (TCE) on the protein in L-02 cells in vitro.</p><p><b>METHODS</b>Thiazolyl blue and Trypan blue tests were used to investigate the cytotoxicity of TCE to L-02 liver cell. The 2-D electrophoresis was used to analyse the expression of proteins in L-02 liver cells. The differentially expressed protein spots were identified by matrix assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF-TOF-MS).</p><p><b>RESULTS</b>When the concentration of TCE exceeded 30 micromol/L, there was distinct cytotoxicity to L-02 cell (P < 0.05). Selected 40 micromol/L to treat L-02 liver cells and analyze the differential proteome expression, the results showed that the expression level of 37 protein spots was up-regulated and 15 protein spots was down-regulated. And 15 proteins were identified by MALDI-TOF-TOF-MS.</p><p><b>CONCLUSION</b>TCE can change the proteome expression of L-02 liver cell. It should provide the fundamental information to identify proteins related to TCE in further study.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Pharmacologie
/
Trichloroéthylène
/
Cartographie peptidique
/
Électrophorèse bidimensionnelle sur gel
/
Protéines
/
Lignée cellulaire
/
Anesthésiques par inhalation
/
Spectrométrie de masse MALDI
/
Protéome
/
Hépatocytes
Limites du sujet:
Humains
langue:
Chinois
Texte intégral:
Chinese Journal of Preventive Medicine
Année:
2005
Type:
Article
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