Molecular basis of Rh DEL phenotype in Zhejiang Han population / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 1029-1032, 2006.
Article
de Zh
| WPRIM
| ID: wpr-282738
Bibliothèque responsable:
WPRO
ABSTRACT
This study was purposed to investigate the molecular basis of Rh DEL phenotype. Rh DEL phenotypes were identified by a serologic adsorption-elution method, the nucleotide sequences of ten RHD exons and exon-intron boundary regions were evaluated by a RHD gene-specific PCR-SSP (PCR-SSP, polymerase chain reaction-sequence specific primer) and sequencing. The results showed that out of 122 random Rh negative donors 35 Rh DEL phenotypes were identified through serologic method, including 6 RhCCdee (17.14%), 28 RhCcdee (80.00%), and 1RhCcdEe (2.86%). Sequence analysis indicated that all DEL phenotypes harbored a RHD 1227 G > A mutation in exon 9. D zygosity test revealed that 29 DEL phenotypes (28 RhCcdee and 1 RhCcdEe) had one RHD gene deleted, and 6 DEL phenotypes (6 RhCCdee) had homogenous RHD gene. It is concluded that RHD 1227A is an important genetic marker for Rh DEL phenotype in Zhejiang Han population.
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Phénotype
/
Polymorphisme génétique
/
Système Rhésus
/
Donneurs de sang
/
Données de séquences moléculaires
/
Séquence nucléotidique
/
Chine
/
Réaction de polymérisation en chaîne
/
Exons
/
Analyse de séquence d'ADN
Type d'étude:
Prognostic_studies
Limites du sujet:
Humans
Pays comme sujet:
Asia
langue:
Zh
Texte intégral:
Journal of Experimental Hematology
Année:
2006
Type:
Article