Establishment of genotyping method for DEL phenotype in Zhejiang Han population / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 1017-1019, 2006.
Article
Dans Zh
| WPRIM
| ID: wpr-282741
Responsable en Bibliothèque :
WPRO
ABSTRACT
In order to establish a genotyping method for DEL phenotype in Zhejiang Han population, an AS-PCR method was developed according to the RHD 1227A allele sequence. Its specificity and sensitivity were assessed in two Rh negative populations whose RHD 1227A or DEL phenotype status was known. The results showed that in evaluation of the method by detecting 50 RHD 1227A positive and 50 RHD 1227A negative individuals, the genotyping method displayed a sensitivity of 100% and a specificity of 100%; in evaluation of the method by detecting 33 DEL positive and 89 DEL negative individuals, the sensitivity was 100%, however, there were two serologically negative samples which were confirmed as positive using genotyping method. After re-testing these two samples with serological method and sequence analysis, it was found that original serological method gave false negative results and genotyping method still showed 100% specificity. The minimal target DNA concentration of this genotyping method is 8.13 ng/microl. In conclusion, designed genotyping method can be used to identify DEL phenotype efficiently in Zhejiang Han Rh negative population.
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Phénotype
/
Polymorphisme génétique
/
Système Rhésus
/
Donneurs de sang
/
Chine
/
Réaction de polymérisation en chaîne
/
Asiatiques
/
Allèles
/
Allergie et immunologie
/
Érythrocytes
Type d'étude:
Prognostic_studies
Limites du sujet:
Adult
/
Female
/
Humans
/
Male
Pays comme sujet:
Asia
langue:
Zh
Texte intégral:
Journal of Experimental Hematology
Année:
2006
Type:
Article