Detection of EWS-FLI1 fusion transcript in Ewing's sarcoma/peripheral primitive neuroectodermal tumors by one-step RT-PCR using paraffin-embedded tissues / 中华病理学杂志
Chinese Journal of Pathology
;
(12): 328-331, 2004.
Article
Dans Chinois
| WPRIM
| ID: wpr-283514
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the expression of EWS-FLI1/ERG fusion transcript resulting from t(11;12)(q24;12) in paraffin-embedded tissues and its diagnostic implication for Ewing's sarcoma/peripheral primitive neuroectodermal tumors (ES/pPNET).</p><p><b>METHODS</b>One-step reverse transcriptase-polymerase chain reaction (RT-PCR) was employed to detect a characteristic EWS-FLI1/ERG fusion transcript in 25 cases of ES/pPNET and 15 cases of other small round cell tumors (including 8 cases of rhabdomyosarcoma, 4 cases of synovial sarcoma, 2 cases of neuroblastoma and 1 case of lymphoma) using formalin-fixed and paraffin-embedded tissues.</p><p><b>RESULTS</b>EWS-FLI1/ERG fusion transcript was detected in 20 of the 25 ES/pPNET cases (80%). The 15 non-ES/pPNET control cases were negative for EWS-FLI1/ERG fusion transcript.</p><p><b>CONCLUSIONS</b>Detection of EWS-FLI1/ERG fusion transcript is a reliable index for molecular diagnosis of ES/pPNET. One-step RT-PCR is a practical method for such analysis in routine paraffin-embedded tumor tissues.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Rhabdomyosarcome
/
Sarcome d'Ewing
/
Facteurs de transcription
/
Protéines de fusion oncogènes
/
Inclusion en paraffine
/
Tumeurs neuroectodermiques primitives périphériques
/
Sarcome synovial
/
RT-PCR
/
Protéine EWS de liaison à l'ARN
/
Diagnostic
Type d'étude:
Etude diagnostique
Limites du sujet:
Adolescent
/
Adulte
/
Femelle
/
Humains
/
Mâle
langue:
Chinois
Texte intégral:
Chinese Journal of Pathology
Année:
2004
Type:
Article
Documents relatifs à ce sujet
MEDLINE
...
LILACS
LIS