Study of mutations in 5'-noncoding region of BCL-6 gene in B-cell non-Hodgkin's lymphomas / 中华病理学杂志
Chinese Journal of Pathology
;
(12): 238-241, 2004.
Article
Dans Chinois
| WPRIM
| ID: wpr-283539
ABSTRACT
<p><b>OBJECTIVE</b>To investigate BCL-6 gene mutations in B-cell non-Hodgkin lymphomas (B-NHL) and their implications in lymphoma pathogenesis.</p><p><b>METHODS</b>Polymerase chain reaction (PCR) and direct DNA sequencing methods were used to identify mutations in the 5'-noncoding region of BCL-6 gene in 135 cases of B-NHL, 5 cases of T-NHL, 5 cases of nodular lymphocyte predominance Hodgkin's lymphoma (NLPHL) and 10 cases of reactive hyperplasia of lymph node.</p><p><b>RESULTS</b>Mutations were identified in 6 cases of nodal DLBCL (27.3%), 4 cases of FL (22.2%), 4 cases of MALT lymphoma (22.2%), 4 cases of extranodal DLBCL (20.7%) and 2 cases of LRH (20%). No mutations were detected in T-NHL and NLPHL (P < 0.05). There were no significant differences in incidences of BCL-6 gene mutations between nodal and extranodal DLBCL (P > 0.05). All mutations were base substitutions and the frequency of single-base change was 0.14 x 10(-2)/bp approximately 0.68 x 10(-2)/bp.</p><p><b>CONCLUSIONS</b>Mutations of the 5'non-coding region of BCL-6 gene may be involved in the pathogenesis and progression of B-NHL. Molecular demonstration of such mutations may provide a marker of lymphomas derived from the germinal center-related B cells.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Anatomopathologie
/
Facteurs de transcription
/
Lymphome malin non hodgkinien
/
Données de séquences moléculaires
/
Séquence nucléotidique
/
Lymphome B
/
Protéines proto-oncogènes
/
Mutation ponctuelle
/
Régions 5' non traduites
/
Protéines de liaison à l'ADN
Type d'étude:
Étude pronostique
Limites du sujet:
Adolescent
/
Adulte
/
Adulte très âgé
/
Aged80
/
Enfant
/
Femelle
/
Humains
/
Mâle
langue:
Chinois
Texte intégral:
Chinese Journal of Pathology
Année:
2004
Type:
Article
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