The effects of nm23-H1 on metastases ability and chemo-sensitivity in Tca8113 cell lines / 华西口腔医学杂志
West China Journal of Stomatology
;
(6): 180-183, 2003.
Article
Dans Chinois
| WPRIM
| ID: wpr-283631
ABSTRACT
<p><b>OBJECTIVE</b>The purpose of this study was to establish a stable, high-efficient and low-toxic way of transfecting nm23-H1 into Tca8113 line cells, and then to find out whether nm23-H1 could affect the invasion and metastases ability of Tca8113 line cells.</p><p><b>METHODS</b>nm23-H1 was transfected into Tca8113 line cells with Lipofect. The different expressions of nm23-H1 between transfected and non-transfected line cells were detected by the method of immunohistochemistry. The difference of the invasion and metastases ability between transfected and non-transfected line cells was detected by transwell-room and wash techniques. The change of chemo-sensitivity was evaluated by MTT.</p><p><b>RESULTS</b>Using pCMV-NEO-BAM system to keep stable expression of nm23-H1, the significant difference of NDPKA expression between transfected and non-transfected Tca8113 line cells was discovered; The metastases ability of transfected Tca8113 line cells decreased significantly; The chemo-sensitivity of transfected Tca8113 line cells to CDDP increased significantly.</p><p><b>CONCLUSION</b>nm23-H1 can inhibit the metastases of Tca8113 line cells and increase the chemo-sensitivity to CDDP significantly.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Anatomopathologie
/
Pharmacologie
/
Tumeurs de la langue
/
Cellules cancéreuses en culture
/
Carcinome épidermoïde
/
Transfection
/
Protéines
/
Régulation de l'expression des gènes tumoraux
/
Cisplatine
/
Nucleoside diphosphate kinase
Type d'étude:
Etude diagnostique
Limites du sujet:
Humains
langue:
Chinois
Texte intégral:
West China Journal of Stomatology
Année:
2003
Type:
Article
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