Study on fingerprints of Citrus aurantium from different places by capillary electrophoresis / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 2362-2364, 2008.
Article
Dans Chinois
| WPRIM
| ID: wpr-283821
ABSTRACT
<p><b>OBJECTIVE</b>To develop a high performance capillary electrophoresis method for Citrus aurantium fingerprints to control its quality.</p><p><b>METHOD</b>The background electrolyte (BGE) was an 80 mmol x L(-1) boric acid solution containing 15 mmol x L(-1) borate. The pH of the BGE was adjusted to 9.70 with KOH solution. The detection wavelength was 201 nm and a voltage of 16 kV was applied. The sample hydrodynamic injection was 0.4 ps with a duration time of 8 sec. C. aurantium was extracted by water and a set of capillary electrophoresis fingerprints (CEFP) containing 12 co-possessing peaks was obtained.</p><p><b>RESULT</b>There were good similarities between the standard CEFP and each set of CEFP of C. aurantium collected from eleven different places, and their similarity coefficients were between 0.973 and 0.996.</p><p><b>CONCLUSION</b>The CEFP has acceptable precision, reproducibility and stability and can be used for the quality control of C. aurantium.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Température
/
Borates
/
Chimie
/
Citrus
/
Électrophorèse capillaire
/
Électrolytes
/
Concentration en ions d'hydrogène
/
Méthodes
langue:
Chinois
Texte intégral:
China Journal of Chinese Materia Medica
Année:
2008
Type:
Article
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