Expression of hypoxia-inducible factor 1α in osteosarcoma and its value in predicting chemosensitivity / 中华肿瘤杂志
Chinese Journal of Oncology
;
(12): 899-904, 2012.
Article
Dans Chinois
| WPRIM
| ID: wpr-284261
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the prognostic values of HIF-1α, APE1, VEGF, and COX-2 protein expressions and their predictive value of tumor necrosis rate and prognosis in osteosarcoma, as well as their interrelationships.</p><p><b>METHODS</b>Formalin-fixed paraffin-embedded tissue samples were obtained from patients with osteosarcoma. Immunohistochemical assay was performed in pre-chemotherapy samples to determine the HIF-1α, VEGF, APE1, and COX-2 protein expression levels. Hematoxylin-eosin staining was used in post-operative samples to determine the tumor necrosis rate. Univariate and multivariate analyses were used to assess the impact of protein expression on prognosis.</p><p><b>RESULTS</b>Tumor tissues were obtained from 49 patients. Their median follow up was 29 months. HIF-1α was significantly correlated to every protein we tested VEGF (P = 0.032), APE1 (P < 0.001), and COX-2 (P < 0.001). HIF-1α protein expression had a significant impact on disease free survival (P = 0.006). Expression of HIF-1α had a sensitivity of 64.7% and a specificity of 71.9% for poor pathological response (< 90% of tumor necrosis) versus good pathological response to chemotherapy (≥ 90% necrosis).</p><p><b>CONCLUSION</b>Expression of HIF-1α is a predictor of tumor response to neoadjuvant chemotherapy and outcome in osteosarcoma and is correlated with VEGF, APE1, and COX-2 expression.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Anatomopathologie
/
Tumeurs osseuses
/
Protocoles de polychimiothérapie antinéoplasique
/
Ostéosarcome
/
Études de suivi
/
Traitement médicamenteux adjuvant
/
Survie sans rechute
/
Traitement néoadjuvant
/
DNA-(apurinic or apyrimidinic site) lyase
/
Facteur de croissance endothéliale vasculaire de type A
Type d'étude:
Étude observationnelle
/
Étude pronostique
/
Facteurs de risque
Limites du sujet:
Adolescent
/
Adulte
/
Adulte très âgé
/
Enfant
/
Femelle
/
Humains
/
Mâle
langue:
Chinois
Texte intégral:
Chinese Journal of Oncology
Année:
2012
Type:
Article
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