Establishment of a new method to induce the differentiation of embryonic pancreatic cells into mature endocrine cells / 中国医学科学院学报
Acta Academiae Medicinae Sinicae
;
(6): 343-347, 2012.
Article
Dans Anglais
| WPRIM
| ID: wpr-284372
ABSTRACT
<p><b>OBJECTIVE</b>To establish a new culture method to induce the differentiation of embryonic pancreatic cells into mature endocrine cells.</p><p><b>METHODS</b>Mouse embryos at day 12.5 were used and embryonic pancreata were isolated. The isolated embryonic pancreata were cultured on the filter for 7 days, which floated in the dish containing medium. During culture, the expression of pancreas duodenum homeobox-1 (PDX-1), a pancreatic stem cell marker, was examined at day 1. The expression of neurogenin 3 (Ngn3), a pancreatic progenitor cell marker, was examined at day 3. The expressions of endocrine and exocrine markers, insulin, glucagon, and carboxypeptidase (CPA) were examined at day 7 by immunohistochemistry. The kinetics of pancreatic marker expression during culture was assayed by real-time PCR.</p><p><b>RESULTS</b>Many pancreatic stem cells still existed in embryonic pancreata cultured for 1 day; meanwhile, these pancreatic stem cells proliferated in high rate. A large amount of pancreatic progenitor cells were found in embryonic pancreata cultured for 3 days.Pancreatic stem/progenitor cells differentiated into mature endocrine and exocrine cells in embryonic pancreata after having been cultured for 7 days. Furthermore, the expression pattern of pancreatic marker is consistent with that in vivo.</p><p><b>CONCLUSION</b>We successfully established a new culture method, with which embryonic pancreatic cells can efficiently differentiate into mature endocrine cell.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Pancréas
/
Différenciation cellulaire
/
Transactivateurs
/
Cellules cultivées
/
Protéines à homéodomaine
/
Techniques de culture cellulaire
/
Biologie cellulaire
/
Embryon de mammifère
/
Facteurs de transcription à motif basique hélice-boucle-hélice
/
Cellules endocrines
Limites du sujet:
Animaux
langue:
Anglais
Texte intégral:
Acta Academiae Medicinae Sinicae
Année:
2012
Type:
Article
Documents relatifs à ce sujet
MEDLINE
...
LILACS
LIS