The prevalence of azoospermia factor microdeletion on the Y chromosome of Chinese infertile men detected by multi-analyte suspension array technology / 亚洲男科学杂志(英文版)
Asian Journal of Andrology
; (6): 873-881, 2008.
Article
de En
| WPRIM
| ID: wpr-284732
Bibliothèque responsable:
WPRO
ABSTRACT
<p><b>AIM</b>To develop a high-throughput multiplex, fast and simple assay to scan azoospermia factor (AZF) region microdeletions on the Y chromosome and establish the prevalence of Y chromosomal microdeletions in Chinese infertile males with azoospermia or oligozoospermia.</p><p><b>METHODS</b>In total, 178 infertile patients with azoospermia (non-obstructed), 134 infertile patients with oligozoospermia as well as 40 fertile man controls were included in the present study. The samples were screened for AZF microdeletion using optimized multi-analyte suspension array (MASA) technology.</p><p><b>RESULTS</b>Of the 312 patients, 36 (11.5%) were found to have deletions in the AZF region. The microdeletion frequency was 14% (25/178) in the azoospermia group and 8.2% (11/134) in the oligospermia group. Among 36 patients with microdeletions, 19 had deletions in the AZFc region, seven had deletions in AZFa and six had deletions in AZFb. In addition, four patients had both AZFb and AZFc deletions. No deletion in the AZF region was found in the 40 fertile controls.</p><p><b>CONCLUSION</b>There is a high prevalence of Y chromosomal microdeletions in Chinese infertile males with azoospermia or oligozoospermia. The MASA technology, which has been established in the present study, provides a sensitive and high-throughput method for detecting the deletion of the Y chromosome. And the results suggest that genetic screening should be advised to infertile men before starting assisted reproductive treatments.</p>
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Oligospermie
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ADN
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Sondes oligonucléotidiques
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Chine
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Épidémiologie
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Reproductibilité des résultats
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Hybridation in situ
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Délétion de gène
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RT-PCR
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Protéines du plasma séminal
Type d'étude:
Prevalence_studies
Limites du sujet:
Adult
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Female
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Humans
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Male
Pays comme sujet:
Asia
langue:
En
Texte intégral:
Asian Journal of Andrology
Année:
2008
Type:
Article