Effect of osthole on bone metabolism in rat femoral tissues in vitro / 中国医学科学院学报
Acta Academiae Medicinae Sinicae
;
(6): 561-566, 2013.
Article
Dans Chinois
| WPRIM
| ID: wpr-285960
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of osthole on bone metabolism in rat femoral tissues in vitro.</p><p><b>METHODS</b>The rat femoral tissues were isolated in vitro. The optimal concentrations of ostehole (1×10(-5) mol/L) and estradiol (1×10(-8) mol/L) (the positive control) were selected by alkaline phosphatase activity (ALP). The ALP and calcium levels were detected by commmerical regents, and the expressions of osteoprotegerin, receptor activator of nuclear factor-κB ligand, runx-related gene 2, and bone morphogenetic protein-2 mRNA were determined by real-time reverse transcription-polymerase chain reaction.</p><p><b>RESULT</b>The osthole (1×10(-5) mol/L) significantly increased the activity of ALP, calcium level as well as the expressions of osteoprotegerin, receptor activator of nuclear factor-κB ligand, runx-related gene-2 and bone morphogenetic protein-2 mRNA in rat femoral tissues in vitro.</p><p><b>CONCLUSION</b>Osthole can improve calcium level and ALP activity and regulate the bone metabolism-related genes in rat femoral tissues.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Pharmacologie
/
Techniques in vitro
/
Calcium
/
Rat Sprague-Dawley
/
Coumarines
/
Phosphatase alcaline
/
Sous-unité alpha 1 du facteur CBF
/
Ligand de RANK
/
Ostéoprotégérine
/
Protéine morphogénétique osseuse de type 2
Limites du sujet:
Animaux
langue:
Chinois
Texte intégral:
Acta Academiae Medicinae Sinicae
Année:
2013
Type:
Article
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