The effects of a hot water soluble extract (S-03) isolated from Isatis indigotica root on influenza A and B viruses in vitro / 病毒学报
Chinese Journal of Virology
;
(6): 218-223, 2011.
Article
Dans Chinois
| WPRIM
| ID: wpr-286051
ABSTRACT
This study was to investigate the antiviral effects of a hot water soluble extract S-03 isolated from Isatis indigotica root on different subtypes of influenza A and B viruses in MDCK cell cultures, using plaque reduction, immunofluorescence and hemo-agglutination inhibition (HAD) assays. Chemical analysis of the extract S-03 showed that it contained high proportion of polysaccharides. The antiviral effects in vitro showed that the S-03 had no effect on different influenza viruses if the drug was used before virus adsorption, but S-03 showed obvious activities against influenza viruses if treatment after virus adsorption or direct reaction of drug and virus before virus adsorption. Hemagglutination inhibition assay showed that S-03 inhibited HA activities of different human influenza viruses (inhibition concentration ranged from 3.12 to 25 mg/mL), avain influenza viruses (inhibition concentration ranged from 25 to 50 mg/mL). The antiviral effects of S-03 on different influenza A and B viruses in vitro might be through the inhibition of the HA to prevent infection.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Virus de la grippe A
/
Virus influenza B
/
Pharmacologie
/
Tests d'inhibition de l'hémagglutination
/
Extraits de plantes
/
Cellules cultivées
/
Chimie
/
Technique d'immunofluorescence
/
Racines de plante
/
Isatis
Limites du sujet:
Animaux
langue:
Chinois
Texte intégral:
Chinese Journal of Virology
Année:
2011
Type:
Article
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