Nicotine reduces endogenous CO concentration and NOS activity in rat corpus cavernosum / 中华男科学杂志
National Journal of Andrology
; (12): 412-415, 2012.
Article
de Zh
| WPRIM
| ID: wpr-286491
Bibliothèque responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To observe the effects of nicotine on endogenous carbon monoxide (CO) concentration and nitric oxide synthase (NOS) activity in the corpus cavernosum of adult male rats, and explore the possible mechanism of cigarette smoking affecting erectile dysfunction.</p><p><b>METHODS</b>Forty adult male rats were equally divided into three treatment groups to receive subcutaneous injection of nicotine at 0.5 mg/kg pre d for 1, 2 and 3 months, and a control group to receive saline only. After treatment, the corpus cavernosum was harvested for detection of CO concentration by modified two-wavelength spectrophotometry and NOS activity by improved Griess measurement.</p><p><b>RESULTS</b>CO concentration and NOS activity were decreased by 9.05 and 13.37%, respectively, after 1 month of nicotine injection (P < 0.01), 16.47 and 22.5% after 2 months (P < 0.01), and 22.99 and 31.74% after 3 months (P < 0.01), as compared with (13.664 +/- 0.404) umol/mg prot and (9.721 +/- 0.470) U/mg prot in the control group.</p><p><b>CONCLUSION</b>Nicotine can reduce endogenous CO concentration and NOS activity in the corpus cavernosum of adult male rats, which suggests the involvement of endogenous CO and NOS in the pathophysiological process of smoking-induced erectile dysfunction .</p>
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Pénis
/
Monoxyde de carbone
/
Fumer
/
Nitric oxide synthase
/
Toxicité
/
Dysfonctionnement érectile
/
Métabolisme
/
Nicotine
Limites du sujet:
Animals
langue:
Zh
Texte intégral:
National Journal of Andrology
Année:
2012
Type:
Article