DNA-PKcs silencing inhibit the DNA repair induced by low dose radiation on human breast epithelial cells / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 727-732, 2009.
Article
Dans Chinois
| WPRIM
| ID: wpr-286650
ABSTRACT
DNA-PKcs, the catalytic subunit of DNA-dependent protein kinase (DNA-PK), plays an important role in the nonhomologous end-joining (NHEJ) pathway of DNA double-strand breaks (DSBs) repair. To investigate the effects of DNA-PKcs down-regulation on cell growth and sensitization to low dose radiation (LDR), we transfected DNA-PKcs siRNA into human mammary epithelia cells MCF10F, then, detected the proliferation curve of the cells and the expression of protiens in DNA repair pathways. The results showed that DNA-PKcs gene silencing, induced by the transfection of DNA-PKcs siRNA could suppress significantly the cell proliferation and inhibit the expression of the DNA repair proteins, such as Ku80, ATM and P53 after 50 cGy 137Cs gamma-irradiation.The results suggested that DNA-PKcs gene silencing could increase the sensitivity of human breast epithelial cells to the LDR, which might be relative with the decrease of the proteins.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Effets des rayonnements
/
Région mammaire
/
Protéines nucléaires
/
Transfection
/
Lignée cellulaire
/
Extinction de l'expression des gènes
/
Biologie cellulaire
/
Petit ARN interférent
/
Réparation de l'ADN
/
Relation dose-effet des rayonnements
Limites du sujet:
Humains
langue:
Chinois
Texte intégral:
Chinese Journal of Biotechnology
Année:
2009
Type:
Article
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