Cloning, expression and functional analysis of the genes in TPS/TPP trehalose synthetic pathway of Meiothermus ruber / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 399-405, 2009.
Article
Dans Chinois
| WPRIM
| ID: wpr-286698
ABSTRACT
By constructing the genomic DNA library of Meiothermus ruber CBS-01, the genes of trehalose phosphate synthase (TPS) and trehalose phosphate phosphatase (TPP) involved in trehalose synthesis were cloned. The genes were cloned into the plasmid pET21a, and expressed in Escherichia coli Rosetta gami (DE3). The activities of these two purified enzymes were confirmed by thin layer chromatography (TLC). Meanwhile, we tested the cellular compatible solutes of M. ruber CBS-01 under different environmental pressure, and found that under hyperosmotic pressure, this strain can accumulate trhalose-6-phosphate, but not trehalose. These results can give more insight to future research in the roles of TPS/TPP and TreS pathway.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Thermus
/
Protéines bactériennes
/
Tréhalose
/
Protéines de fusion recombinantes
/
Clonage moléculaire
/
Phosphoric monoester hydrolases
/
Escherichia coli
/
Génétique
/
Glucosyltransferases
/
Métabolisme
langue:
Chinois
Texte intégral:
Chinese Journal of Biotechnology
Année:
2009
Type:
Article
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