A comparative study on three models of co-culture of neurons and astrocytes / 中国当代儿科杂志
Chinese Journal of Contemporary Pediatrics
;
(12): 984-987, 2010.
Article
Dans Chinois
| WPRIM
| ID: wpr-286930
ABSTRACT
<p><b>OBJECTIVE</b>To find a better method for harvesting highly purified neurons by comparing three methods used for co-culture of neurons and astrocytes.</p><p><b>METHODS</b>The co-culture models of neurons and astrocytes were established by primary culture, Banker's co-culture method or Transwell cell-culture inserts. The neurons and astrocytes cultured in vitro were from neonatal rats.</p><p><b>RESULTS</b>The highly purified neurons were not harvested by primary culture because the neurons and astrocytes grew on the same cover slip and it was difficult to control the growth velocity of astrocytes. The highly purified neurons were harvested by Banker's co-culture method or the method using Transwell cell-culture inserts, but the procedure of the former was more complicated than that of the later.</p><p><b>CONCLUSIONS</b>The culture method using Transwell cell-culture inserts is recommended for the establishment of the co-culture system of neurons and astrocytes.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Cellules cultivées
/
Astrocytes
/
Techniques de coculture
/
Biologie cellulaire
/
Neurones
Limites du sujet:
Animaux
langue:
Chinois
Texte intégral:
Chinese Journal of Contemporary Pediatrics
Année:
2010
Type:
Article
Documents relatifs à ce sujet
MEDLINE
...
LILACS
LIS