Determination of homocysteine level in plasma by high performance liquids chromatography with fluorescence detection / 中国应用生理学杂志

ABSTRACT

<p><b>AIM</b>To establish a method for detection of plasma total homocysteine with HPLC.</p><p><b>METHODS</b>The chromatography analysis was carried out using a Symmetry Shield RP18. The mobile phase was sodium acetate (0.08 mol/L) and methanol (1%) and we utilized a HPLC system with fluorescence detection of plasma homocysteine derivatized from reaction with 7-fluorobenzo-2-oxa-1,3-diazole-4-sulphonate (SBD-F).</p><p><b>RESULTS</b>The average recoveries were 95.8 - 100.8% and the relative standard deviations were 1.2-2.0%.</p><p><b>CONCLUSION</b>The results showed it to be a rapid and accurate method for the determination of homocysteine level in plasma.</p>