Cloning and sequencing of alpha, beta globin coding genes in Tibetans living at high altitude / 中国应用生理学杂志
Chinese Journal of Applied Physiology
;
(6): 196-199, 2005.
Article
Dans Chinois
| WPRIM
| ID: wpr-287058
ABSTRACT
<p><b>AIM</b>To explore the molecular biological mechanism of hemoglobin with high oxygen affinity in Tibetans by determining the sequence of globin cDNA in Tibetans living at high altitude.</p><p><b>METHODS</b>Total RNA was isolated from human bone marrow samples of three Tibetans who live in Qinghai-Tibet plateau. cDNA fragments coding for alpha and beta genes of human hemoglobin were obtained through RT-PCR and were ligated to plasmid pGEM-T easy vectors, and then the ligation liquid were transformed to Escherichia coli and cloned and sequenced. Nucleotide sequences were compared with GenBank data by BLAST method.</p><p><b>RESULTS</b>sequence of a globin cDNA in Tibetans were the same with the registering globin genes in the GenBank, and Hb Abruzzo (beta143 (H21), His- > Arg) gene mutation, a high oxygen affinity beta globin mutation, was found in one Tibetan' beta goblin coding gene (CAC- > CGC).</p><p><b>CONCLUSION</b>This hemoglobin gene mutation may be associated with high altitude adaptation of Tibetans living at high altitude.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Hémoglobines anormales
/
ARN
/
Données de séquences moléculaires
/
Clonage moléculaire
/
Analyse de séquence d'ADN
/
ADN complémentaire
/
Tibet
/
Asiatiques
/
Altitude
/
Globines alpha
Limites du sujet:
Adulte
/
Humains
/
Mâle
Pays comme sujet:
Asie
langue:
Chinois
Texte intégral:
Chinese Journal of Applied Physiology
Année:
2005
Type:
Article
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