Effect of acrosin inhibitor KF-950 on acrosin activity and acrosome of human sperm / 中华男科学杂志

ABSTRACT

<p><b>OBJECTIVES</b>To study the inhibitory effect of KF-950 on human acrosin and sperm acrosome.</p><p><b>METHODS</b>Human acrosin was extracted and purified with 2% acetic acid, and its residual activity was evaluated by BAEE/ADH assay after treated with different concentrations of KF-950. ABC assay was used to observe the effect of KF-950 on human acrosome with Biotin-PSA as a probe.</p><p><b>RESULTS</b>1. The activity of normal sperm acrosin was (37.65 +/- 4.47) U/L. 2. The residual activity was inversely related to the concentration of KF-950 (r = -0.998), and had a dose-response curve. The result could be described by Y = 7.57-1.895X. 3. With increase of KF-950 concentration and prolongation of action time, the staining rate of acrosome obviously dropped (P < 0.01).</p><p><b>CONCLUSIONS</b>KF-950 directly inhibits acrosin activity and assumely injures sperm acrosome. It might be a new kind of highly effective inhibitor.</p>