Effects of cyclosporin A on expression of FasL and Fas in the contralateral testis after the unilateral testis injured in KM mouse / 中华男科学杂志

ABSTRACT

<p><b>OBJECTIVES</b>To investigate the effects of Cyclosporin A (CsA) on spermatogenesis and expression of FasL and Fas in the contralateral testis after the unilateral testis was injured.</p><p><b>METHODS</b>60 mice were randomly divided equally into groups A (control), B (the unilateral testis was injured by glacial acetic acid), C (excision of ipsilateral testis at 6 hours after the unilateral testis was injured by glacial acetic acid) and D (CsA within 6 hours after the unilateral testis was injured by glacial acetic acid). Sperm density and sperm motility were evaluated after 4 weeks. Expression of FasL and Fas was performed by immunohistochemistry (SP method). The positive cells with SP staining in seminiferous tubules were calculated.</p><p><b>RESULTS</b>Sperm density and sperm motility in group D were significantly increased compared with group B(P < 0.05). Expression of FasL and Fas in group D decreased significantly compared with group B (24.3 +/- 7.0 vs 37.8 +/- 5.8 and 17.8 +/- 4.3 vs 32.4 +/- 3.6, P < 0.05).</p><p><b>CONCLUSIONS</b>CsA decreased expression of Fas and FasL and maintained spermatogenesis in the contralateral testis after the unilateral testis was injured by glacial acetic acid.</p>