Effect of Kanglaite injection oncyclooxygenase activity in lung carcinoma A549 cell / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To study the effect of Kanglaite injection on cyclooxygenase activity in lung carcinoma A549 cells.</p><p><b>METHODS</b>The expression of mRNA of COX-1 and COX-2 were measured by RT-PCR. The expression of COX-2 protein was measured by Western-blot.</p><p><b>RESULT</b>Kanglaite injection could selectively decreased COX-2 mRNA expression and protein expression while COX-1 mRNA expression was unchanged.</p><p><b>CONCLUSION</b>Kanglaite injection is selectively inhibitory agent of COX-2, and possess inhibitory effects on cyclooxygenase 2.</p>